GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345214/primaryOK200TranscriptomicsCaenorhabditis elegansExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345214GEOGSEfalseEctopic expression of the germline transcription factor LSL-1 contributes to developmental delay following failed maternal epigenetic reprogrammingProper development requires mechanisms that prevent germline genes from being expressed in non-germline tissues. In this study, we investigated how the germline transcription factor LSL-1 contributes to developmental defects caused by failed maternal epigenetic reprogramming in Caenorhabditis elegans. We found that LSL-1 becomes ectopically expressed in somatic tissues of spr-5; met-2 mutants and that depletion of LSL-1 partially rescues their developmental delay. RNA-seq analyses showed extensive overlap between MES-4- and LSL-1-dependent transcriptional programs and revealed that ectopic lsl-1 expression itself depends on MES-4. These findings identify LSL-1 as a downstream effector of MES-4 that promotes aberrant germline-associated transcription and developmental delay.2026/09/04GSE345214GSM9999609GSM9999618GSM9999619GSM9999616GSM9999617GSM9999614GSM9999615GSM9999612GSM9999613GSM9999610GSM9999611GSM999962032326345214Caenorhabditis elegans