GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345409/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345409GEOGSEfalseCD39-stratified CD8+ tumor-infiltrating lymphocytes from head and neck squamous cell carcinoma profiled by bulk RNA sequencing after ex vivo anti-PD-1 and polydatin treatmentTumor-infiltrating lymphocytes were recovered from six untreated head and neck squamous cell carcinoma surgical specimens and pooled. CD8+ TILs were sorted by flow cytometry into CD39-negative, CD39-medium and CD39-high populations and cultured for 72 h under four conditions: vehicle only, anti-PD-1 (nivolumab), polydatin (a G6PD inhibitor), and the combination of anti-PD-1 and polydatin. Bulk RNA sequencing of the twelve resulting libraries was used to determine how CD39 expression level stratifies the transcriptional response to combined checkpoint blockade and metabolic inhibition.2026/09/21GSE345409GSM10002838GSM10002837GSM10002836GSM10002846GSM10002835GSM10002839GSM10002841GSM10002840GSM10002845GSM10002844GSM10002843GSM1000284234281345409Homo sapiens