{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345454/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345454"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Human three-dimensional engineered muscle tissue characterization and intramyocellular lipid modeling","description":"Three-dimensional engineered muscle tissues (EMTs) are transformative tools for modeling skeletal muscle physiology and pathology in vitro. Here, we perform a comprehensive comparison of EMTs derived from primary human myoblasts (hP-Myo) and hiPS-derived myoblasts (hiPS-Myo) to evaluate their structural, functional, and transcriptional characteristics. Contractile performance was quantified using a magnetic force-sensing platform, revealing that hP-Myo EMTs generate ~2 fold higher twitch forces and enhanced tetanic responses compared to hiPS-Myo EMTs. Tissue architecture and maturation were assessed and demonstrated significant larger myofiber diameters in hP-Myo EMTs. Transcriptomic profiling highlighted that hP-Myo EMTs maintain a mature skeletal muscle-like signature, marked by enriched pathways linked to sarcomere organization and fast-/slow-twitch fiber specification. To model metabolic dysfunction, hiPS-Myo EMTs were subjected to lipid overload, recapitulating hallmarks of intracellular lipid (IMCL) accumulation, including impaired contractility, blunted force-frequency responses, and dysregulated lipid metabolism genes.","dates":{"publication":"2026/08/31"},"accession":"GSE345454","cross_references":{"GSM":["GSM10003990","GSM10003991","GSM10003992","GSM10003906","GSM10003907","GSM10003908","GSM10003909","GSM10003903","GSM10003904","GSM10003905","GSM10003986","GSM10003987","GSM10003988","GSM10003989","GSM10004037","GSM10003982","GSM10003983","GSM10004038","GSM10003984","GSM10003985","GSM10004033","GSM10004034","GSM10003980","GSM10004035","GSM10004036","GSM10003981","GSM10004030","GSM10004031","GSM10004032","GSM10003979","GSM10003975","GSM10003976","GSM10003977","GSM10003978","GSM10003971","GSM10004026","GSM10003972","GSM10004027","GSM10003973","GSM10004028","GSM10003974","GSM10004029","GSM10003928","GSM10003929","GSM10003924","GSM10003925","GSM10003926","GSM10003927","GSM10003920","GSM10003921","GSM10003922","GSM10003923","GSM10003917","GSM10003918","GSM10003919","GSM10003913","GSM10003914","GSM10003915","GSM10003916","GSM10003997","GSM10003910","GSM10003998","GSM10003999","GSM10003911","GSM10003912","GSM10003993","GSM10003994","GSM10003995","GSM10003996","GSM10004000","GSM10004001","GSM10004002","GSM10004003","GSM10003946","GSM10003947","GSM10003948","GSM10003949","GSM10003942","GSM10003943","GSM10003944","GSM10003945","GSM10003940","GSM10003941","GSM10003939","GSM10003935","GSM10003936","GSM10003937","GSM10003938","GSM10003931","GSM10003932","GSM10003933","GSM10003934","GSM10003930","GSM10004022","GSM10004023","GSM10004024","GSM10003970","GSM10004025","GSM10004020","GSM10004021","GSM10003968","GSM10003969","GSM10004019","GSM10003964","GSM10003965","GSM10003966","GSM10003967","GSM10003960","GSM10004015","GSM10004016","GSM10003961","GSM10004017","GSM10003962","GSM10003963","GSM10004018","GSM10004011","GSM10004012","GSM10004013","GSM10004014","GSM10004010","GSM10003957","GSM10003958","GSM10003959","GSM10003953","GSM10004008","GSM10003954","GSM10004009","GSM10003955","GSM10003956","GSM10004004","GSM10003950","GSM10004005","GSM10003951","GSM10004006","GSM10004007","GSM10003952"],"GPL":["34281"],"GSE":["345454"],"taxon":["Homo sapiens"],"PMID":["[41163722]"]}}