GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345454/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345454GEOGSEfalseHuman three-dimensional engineered muscle tissue characterization and intramyocellular lipid modelingThree-dimensional engineered muscle tissues (EMTs) are transformative tools for modeling skeletal muscle physiology and pathology in vitro. Here, we perform a comprehensive comparison of EMTs derived from primary human myoblasts (hP-Myo) and hiPS-derived myoblasts (hiPS-Myo) to evaluate their structural, functional, and transcriptional characteristics. Contractile performance was quantified using a magnetic force-sensing platform, revealing that hP-Myo EMTs generate ~2 fold higher twitch forces and enhanced tetanic responses compared to hiPS-Myo EMTs. Tissue architecture and maturation were assessed and demonstrated significant larger myofiber diameters in hP-Myo EMTs. Transcriptomic profiling highlighted that hP-Myo EMTs maintain a mature skeletal muscle-like signature, marked by enriched pathways linked to sarcomere organization and fast-/slow-twitch fiber specification. To model metabolic dysfunction, hiPS-Myo EMTs were subjected to lipid overload, recapitulating hallmarks of intracellular lipid (IMCL) accumulation, including impaired contractility, blunted force-frequency responses, and dysregulated lipid metabolism genes.2026/08/31GSE345454GSM10003990GSM10003991GSM10003992GSM10003906GSM10003907GSM10003908GSM10003909GSM10003903GSM10003904GSM10003905GSM10003986GSM10003987GSM10003988GSM10003989GSM10004037GSM10003982GSM10003983GSM10004038GSM10003984GSM10003985GSM10004033GSM10004034GSM10003980GSM10004035GSM10004036GSM10003981GSM10004030GSM10004031GSM10004032GSM10003979GSM10003975GSM10003976GSM10003977GSM10003978GSM10003971GSM10004026GSM10003972GSM10004027GSM10003973GSM10004028GSM10003974GSM10004029GSM10003928GSM10003929GSM10003924GSM10003925GSM10003926GSM10003927GSM10003920GSM10003921GSM10003922GSM10003923GSM10003917GSM10003918GSM10003919GSM10003913GSM10003914GSM10003915GSM10003916GSM10003997GSM10003910GSM10003998GSM10003999GSM10003911GSM10003912GSM10003993GSM10003994GSM10003995GSM10003996GSM10004000GSM10004001GSM10004002GSM10004003GSM10003946GSM10003947GSM10003948GSM10003949GSM10003942GSM10003943GSM10003944GSM10003945GSM10003940GSM10003941GSM10003939GSM10003935GSM10003936GSM10003937GSM10003938GSM10003931GSM10003932GSM10003933GSM10003934GSM10003930GSM10004022GSM10004023GSM10004024GSM10003970GSM10004025GSM10004020GSM10004021GSM10003968GSM10003969GSM10004019GSM10003964GSM10003965GSM10003966GSM10003967GSM10004015GSM10003960GSM10004016GSM10003961GSM10004017GSM10003962GSM10003963GSM10004018GSM10004011GSM10004012GSM10004013GSM10004014GSM10004010GSM10003957GSM10003958GSM10003959GSM10003953GSM10004008GSM10003954GSM10004009GSM10003955GSM10003956GSM10004004GSM10003950GSM10004005GSM10003951GSM10004006GSM10004007GSM1000395234281345454Homo sapiens[41163722]