{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345456/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345456"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"MiR-9-3 promotes osteoblast differentiation of bone marrow mesenchymal stem cells by targeting Smoc2","description":"MicroRNAs are critical regulators of osteoblast differentiation and bone formation. In this study, we reported that miR-9-3 regulate osteoblast differentiation and bone formation by targeting SPARC-related modular calcium binding 2 (Smoc2). MiR-9-3 deletion led to reduced size and postweaning lethality in 10% of the mice. Using microcomputed tomography, we found that mice lacking miR-9-3 exhibited decreased trabecular bone mass and bone volume. Importantly, the serum level of PINP and OCN, both markers of bone formation, was also significantly decreased in miR-9-3-deficient mice. Deletion of miR-9-3 in mice did not affect osteoclast differentiation but impaired mesenchymal stem cells (MSCs)-mediated osteoblast differentiation and mineralization, as evidenced by reduced ALP and Alizarin Red S staining. RNA sequencing revealed reduced osteoblast differentiation and bone morphogenetic proteins (BMPs) signaling in miR-9-3-deficient MSCs. Mechanistically, dual-luciferase reporter assays identified Smoc2, an antagonist of BMP, as the direct target of miR-9-3. Moreover, miR-9-3 deficiency led to Smoc2 upregulation, which subsequently inhibited BMP/Smad pathway in MSCs. Collectively, our results reveal that Smoc2, a repressor of BMP signaling, is directly targeted and suppressed by miR-9-3, and loss of miR-9-3 increases Smoc2 expression, thereby reducing BMP signaling and impairing osteoblast differentiation.","dates":{"publication":"2026/09/03"},"accession":"GSE345456","cross_references":{"GSM":["GSM10004047","GSM10004051","GSM10004052","GSM10004050","GSM10004048","GSM10004049"],"GPL":["34290"],"GSE":["345456"],"taxon":["Mus musculus"]}}