{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345888/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345888"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Cellular and molecular mechanisms determining allogeneic CAR T cell rejection and expansion -- Submission 1 of 2 (Bulk TCR)","description":"Allogeneic CAR T cells could overcome limitations of autologous therapies but are limited by immune rejection. We evaluated 11 patients with large B-cell lymphoma treated with a single lot of cemacabtagene ansegedleucel (cema-cel), an allogeneic anti-CD19 CAR T product. Despite receiving identical infusion products, patients exhibited heterogeneous cema-cel expansion and clinical outcomes. Our integrated analyses using longitudinal TCRβ sequencing, single-cell molecular profiling, and mixed lymphocyte reaction assays revealed that high frequencies of pre-existing, recipient-derived alloreactive CD8+ T cells mediated rapid CAR T rejection in non-expanders. Furthermore, effector-like features, rather than stem/central memory programs, drove robust clonal CAR T expansion consistently across expanders. We confirmed similar expansion patterns in two independent cohorts treated with a separate lot of cema-cel or an allogeneic anti-BCMA CAR T product. These findings highlight distinct cell-extrinsic and cell-intrinsic mechanisms that influence allogeneic CAR T performance and provide insights to optimize donor selection, manufacturing, and product design.","dates":{"publication":"2026/09/23"},"accession":"GSE345888","cross_references":{"GSM":["GSM10018568","GSM10018601","GSM10018569","GSM10018602","GSM10018575","GSM10018576","GSM10018577","GSM10018578","GSM10018571","GSM10018572","GSM10018573","GSM10018574","GSM10018570","GSM10018539","GSM10018579","GSM10018537","GSM10018538","GSM10018586","GSM10018542","GSM10018543","GSM10018587","GSM10018588","GSM10018544","GSM10018545","GSM10018589","GSM10018582","GSM10018583","GSM10018540","GSM10018584","GSM10018585","GSM10018541","GSM10018580","GSM10018581","GSM10018546","GSM10018547","GSM10018548","GSM10018549","GSM10018597","GSM10018553","GSM10018554","GSM10018598","GSM10018599","GSM10018555","GSM10018556","GSM10018593","GSM10018594","GSM10018550","GSM10018551","GSM10018595","GSM10018596","GSM10018552","GSM10018590","GSM10018591","GSM10018592","GSM10018557","GSM10018558","GSM10018559","GSM10018564","GSM10018565","GSM10018566","GSM10018600","GSM10018567","GSM10018560","GSM10018561","GSM10018562","GSM10018563"],"GPL":["30173","24676"],"GSE":["345888"],"taxon":["Homo sapiens"],"PMID":["[42788887]"]}}