{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345930/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Drosophila melanogaster"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345930"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Splicing Suppression Alters STING Transcription Start Site to Attenuate Antiviral Innate Immunity [CrPV vs mi]","description":"The cGAS (cyclic GMP-AMP synthase) -STING (Stimulator of interferon genes) pathway plays a central role in antiviral innate immunity in vertebrates. Recent studies have shown that invertebrates possess a related cGLR (cGAS-like receptor)-STING pathway. However, due to the lack of direct evidence demonstrating viral evasion of cGLR-STING-mediated immunity, the functional importance of cGLR-STING pathway in antiviral responses remains uncertain in invertebrates. Here, we show that an insect picorna-like virus CrPV (Cricket Paralysis virus) inhibits STING-mediated antiviral activity by altering the transcription start site of STING. Mechanistically, CrPV infection down-regulates several spliceosomal genes and disrupts pre-mRNA splicing. This splicing impairment is sufficient to stall RNA polymerase II in transcriptional initiation condensates, reduce global transcription efficiency, and reprogram the transcription start site of STING. Such a shift diverts STING expression from a functional antiviral isoform to a non-antiviral variant that partially suppresses STING activity, thereby disabling the cGLR-STING pathway. Our findings uncover an unconventional viral immune evasion strategy and an unexpected link between splicing suppression and STING-mediated antiviral response. Given the evolutionary conservation of splicing mechanisms and STING-like pathways, this mechanism may represent a broadly applicable regulatory principle in immune defense across species.","dates":{"publication":"2026/09/07"},"accession":"GSE345930","cross_references":{"GSM":["GSM10019489","GSM10019488","GSM10019487","GSM10019492","GSM10019491","GSM10019490"],"GPL":["25244"],"GSE":["345930"],"taxon":["Drosophila melanogaster"]}}