GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346015/primary200OKTranscriptomicsDanio rerioExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346015GEOGSEfalseSingle-nuclei RNA-Seq to characterize the immediate whole-animal postprandial transcriptional response following a high-fat meal in larval zebrafishIn this study, we employed a fluorescent BODIPY-fatty acid feeding assay and 10x Genomics single nuclei RNA-sequencing (snRNA-seq) to explore the simultaneous transcriptional responses following consumption of a high-fat meal in the context of a whole vertebrate organism (larval zebrafish). We used the whole-animal nuclear transcriptome sampling over short sampling periods (0 to 4 hours) to demonstrate how known metabolic pathways intertwine in new functional/spatial and immediate temporal contexts, and demonstrated the power of larval zebrafish snRNA-seq and fluorescent high-fat feeding protocols to describe the whole-animal response to consumption of a high-fat meal. To our knowledge, such an approach has never been accomplished before in a vertebrate model.2026/09/04GSE346015GSM10021501GSM10021512GSM10021500GSM10021511GSM10021510GSM10021499GSM10021509GSM10021508GSM10021507GSM10021506GSM10021505GSM10021504GSM10021503GSM10021513GSM1002150237311346015Danio rerio