{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346097/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Equus caballus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346097"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Circulating Cell-Free RNA Reflects Immune-Associated and Airway Remodeling Signatures in Equine Asthma","description":"This study presents the first comprehensive transcriptomic analysis of plasma cell-free RNA (cfRNA) in equine asthma (EA), a clinically relevant translational model for human asthma. We profiled cfRNA from 16 EA cases (including mastocytic, neutrophilic, and paucigranulocytic phenotypes) and 12 healthy controls using cfRNA-stabilized blood collection and UMI-based total RNA-seq optimized for low-input samples. For comparative molecular profiling, we sequenced bronchoalveolar lavage (BAL) fluid RNA (n=12: 7 EA, 5 controls) and whole blood RNA (n=11: 6 EA, 5 controls) from partially matched individuals, with 9 horses providing complete three-compartment sampling. RNA-seq libraries were prepared using the SMARTer Stranded Total RNA-Seq Pico Input Kit with UMIs (Takara Bio, v3/v4) and sequenced as paired-end 150 bp reads on an Illumina NovaSeq X platform. Our analysis revealed that cfRNA captures biologically relevant inflammatory and airway remodeling signatures characteristic of asthma, including altered immune cell composition and upregulation of key alarmins and remodeling-associated transcripts. These results establish cfRNA as a sensitive complementary approach for characterizing molecular perturbations in asthma pathogenesis and highlight its potential for biomarker discovery across inflammatory and allergic airway diseases.","dates":{"publication":"2026/09/04"},"accession":"GSE346097","cross_references":{"GSM":["GSM10024570","GSM10024571","GSM10024572","GSM10024573","GSM10024563","GSM10024564","GSM10024565","GSM10024566","GSM10024600","GSM10024567","GSM10024568","GSM10024601","GSM10024569","GSM10024580","GSM10024581","GSM10024582","GSM10024583","GSM10024584","GSM10024574","GSM10024575","GSM10024576","GSM10024577","GSM10024578","GSM10024579","GSM10024590","GSM10024591","GSM10024592","GSM10024593","GSM10024594","GSM10024551","GSM10024595","GSM10024585","GSM10024586","GSM10024587","GSM10024588","GSM10024589","GSM10024560","GSM10024561","GSM10024562","GSM10024596","GSM10024552","GSM10024597","GSM10024553","GSM10024554","GSM10024598","GSM10024599","GSM10024555","GSM10024556","GSM10024557","GSM10024558","GSM10024559"],"GPL":["36047"],"GSE":["346097"],"taxon":["Equus caballus"],"PMID":["[42644703]"]}}