{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346252/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Toxoplasma gondii"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346252"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomic profiling of Toxoplasma gondii eIF5A K49 mutants during alkaline stress-induced differentiation","description":"This study investigates how the acetylation status of lysine 49 (K49) in eukaryotic translation initiation factor 5A (eIF5A) influences transcriptional heterogeneity and developmental-state transitions in Toxoplasma gondii. Using the ME49 strain as the genetic background, we performed 10x Genomics single-cell RNA sequencing on wild-type parasites, an eIF5A-K49Q acetylation-mimic mutant, and an eIF5A-K49R non-acetylatable charge-conservative mutant. Each parasite line was cultured under standard conditions or exposed to alkaline medium (pH 8.2 for 4 days) to induce tachyzoite-to-bradyzoite differentiation in vitro. For each genotype-by-condition group, three independently cultured biological replicates were pooled before single-cell capture to generate one scRNA-seq library. Six pooled libraries were generated in total. This dataset enables characterization of parasite cell-state heterogeneity and transcriptional programs associated with eIF5A K49 status during stress-induced differentiation.","dates":{"publication":"2026/09/05"},"accession":"GSE346252","cross_references":{"GSM":["GSM10031102","GSM10031101","GSM10031098","GSM10031097","GSM10031100","GSM10031099"],"GPL":["26742"],"GSE":["346252"],"taxon":["Toxoplasma gondii"]}}