GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346252/primaryOK200TranscriptomicsToxoplasma gondiiExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346252GEOGSEfalseSingle-cell transcriptomic profiling of Toxoplasma gondii eIF5A K49 mutants during alkaline stress-induced differentiationThis study investigates how the acetylation status of lysine 49 (K49) in eukaryotic translation initiation factor 5A (eIF5A) influences transcriptional heterogeneity and developmental-state transitions in Toxoplasma gondii. Using the ME49 strain as the genetic background, we performed 10x Genomics single-cell RNA sequencing on wild-type parasites, an eIF5A-K49Q acetylation-mimic mutant, and an eIF5A-K49R non-acetylatable charge-conservative mutant. Each parasite line was cultured under standard conditions or exposed to alkaline medium (pH 8.2 for 4 days) to induce tachyzoite-to-bradyzoite differentiation in vitro. For each genotype-by-condition group, three independently cultured biological replicates were pooled before single-cell capture to generate one scRNA-seq library. Six pooled libraries were generated in total. This dataset enables characterization of parasite cell-state heterogeneity and transcriptional programs associated with eIF5A K49 status during stress-induced differentiation.2026/09/05GSE346252GSM10031102GSM10031101GSM10031098GSM10031097GSM10031100GSM1003109926742346252Toxoplasma gondii