{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346274/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Rattus norvegicus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346274"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"BRD4 maintains chromaffin-cell identity and promotes pheochromocytoma progression through a SMAD2-dependent transcriptional program","description":"To determine whether BRD4 directly regulates chromaffin-cell identity genes at the chromatin level, we performed CUT&Tag (Cleavage Under Targets and Tagmentation) using a BRD4-specific antibody in PC12 cells, in parallel with chromatin immunoprecipitation (ChIP). This approach allowed genome-wide mapping of BRD4 occupancy. Our CUT&Tag and ChIP analyses revealed that BRD4 directly occupies the regulatory regions of key chromaffin-cell genes, including TH, CHGB, and PHOX2A. These findings demonstrate that BRD4 acts as a direct transcriptional regulator of genes essential for chromaffin-cell identity and catecholamine biosynthesis, providing a mechanistic basis for its role in maintaining chromaffin-cell function and pheochromocytoma progression.","dates":{"publication":"2026/09/10"},"accession":"GSE346274","cross_references":{"GSM":["GSM10031550","GSM10031549"],"GPL":["37481"],"GSE":["346274"],"taxon":["Rattus norvegicus"]}}