GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346274/primaryOK200GenomicsRattus norvegicusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346274GEOGSEfalseBRD4 maintains chromaffin-cell identity and promotes pheochromocytoma progression through a SMAD2-dependent transcriptional programTo determine whether BRD4 directly regulates chromaffin-cell identity genes at the chromatin level, we performed CUT&Tag (Cleavage Under Targets and Tagmentation) using a BRD4-specific antibody in PC12 cells, in parallel with chromatin immunoprecipitation (ChIP). This approach allowed genome-wide mapping of BRD4 occupancy. Our CUT&Tag and ChIP analyses revealed that BRD4 directly occupies the regulatory regions of key chromaffin-cell genes, including TH, CHGB, and PHOX2A. These findings demonstrate that BRD4 acts as a direct transcriptional regulator of genes essential for chromaffin-cell identity and catecholamine biosynthesis, providing a mechanistic basis for its role in maintaining chromaffin-cell function and pheochromocytoma progression.2026/09/10GSE346274GSM10031550GSM1003154937481346274Rattus norvegicus