GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346716/primaryOK200OtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346716GEOGSEfalseNWU Study 3- SLAM-seq timecourse of KOLF2.2J hiPSC after Auxin-induced protein degradation of specific gene productsThis study investigates the impact of gene products on nscent transcription in growing hiPSC at 0, 6 & 24 hours after acute protein depletion. Crispr editing was used to add an auxin-dependent degron to the C-termini of gene products expressed from the endogeneous loci and to insert an auxin-responsive degradation E3 ligase to the AAVS locus. Rapid (<30 min), specific target protein degradation in KOLF2.2J hiPSCs was induced by a synthetic auxin derivative (5-Ad-IAA) and bulk ATAC-seq was performed at timepoints to define early (6 hr) and later (24 hr) downstream transcriptional dependencies.2026/09/12GSE346716GSM10039304GSM10039303GSM10039306GSM10039305GSM10039308GSM10039307GSM10039300GSM10039302GSM10039301GSM10039098GSM10039097GSM10039099GSM10039090GSM10039092GSM10039091GSM10039094GSM10039093GSM10039096GSM10039095GSM10039087GSM10039086GSM10039089GSM10039088GSM10039081GSM10039080GSM10039083GSM10039082GSM10039085GSM10039084GSM10039197GSM10039076GSM10039075GSM10039196GSM10039199GSM10039078GSM10039198GSM10039077GSM10039079GSM10039191GSM10039070GSM10039190GSM10039072GSM10039193GSM10039192GSM10039071GSM10039195GSM10039074GSM10039194GSM10039073GSM10039186GSM10039065GSM10039185GSM10039064GSM10039188GSM10039067GSM10039066GSM10039187GSM10039069GSM10039189GSM10039068GSM10039180GSM10039182GSM10039061GSM10039060GSM10039181GSM10039063GSM10039184GSM10039183GSM10039062GSM10039175GSM10039054GSM10039296GSM10039295GSM10039174GSM10039053GSM10039298GSM10039177GSM10039056GSM10039297GSM10039176GSM10039055GSM10039179GSM10039058GSM10039178GSM10039057GSM10039299GSM10039059GSM10039290GSM10039292GSM10039171GSM10039050GSM10039291GSM10039170GSM10039052GSM10039294GSM10039173GSM10039172GSM10039051GSM10039293GSM10039039GSM10039043GSM10039285GSM10039164GSM10039163GSM10039042GSM10039284GSM10039166GSM10039045GSM10039287GSM10039286GSM10039165GSM10039044GSM10039289GSM10039168GSM10039047GSM10039046GSM10039288GSM10039167GSM10039049GSM10039169GSM10039048GSM10039281GSM10039160GSM10039280GSM10039283GSM10039162GSM10039041GSM10039040GSM10039282GSM10039161GSM10039029GSM10039149GSM10039032GSM10039274GSM10039153GSM10039152GSM10039031GSM10039273GSM10039155GSM10039034GSM10039276GSM10039275GSM10039154GSM10039033GSM10039278GSM10039157GSM10039036GSM10039277GSM10039156GSM10039035GSM10039159GSM10039038GSM10039158GSM10039037GSM10039279GSM10039270GSM10039272GSM10039151GSM10039030GSM10039271GSM10039150GSM10039139GSM10039259GSM10039138GSM10039263GSM10039142GSM10039262GSM10039141GSM10039265GSM10039144GSM10039143GSM10039264GSM10039146GSM10039267GSM10039266GSM10039145GSM10039269GSM10039148GSM10039268GSM10039147GSM10039261GSM10039140GSM10039260GSM10039249GSM10039128GSM10039248GSM10039127GSM10039129GSM10039252GSM10039131GSM10039251GSM10039130GSM10039254GSM10039133GSM10039132GSM10039253GSM10039135GSM10039256GSM10039255GSM10039134GSM10039258GSM10039137GSM10039257GSM10039136GSM10039250GSM10039238GSM10039117GSM10039237GSM10039116GSM10039119GSM10039239GSM10039118GSM10039241GSM10039120GSM10039240GSM10039243GSM10039122GSM10039242GSM10039121GSM10039245GSM10039124GSM10039123GSM10039244GSM10039126GSM10039247GSM10039246GSM10039125GSM10039106GSM10039227GSM10039226GSM10039105GSM10039229GSM10039108GSM10039228GSM10039107GSM10039109GSM10039230GSM10039232GSM10039111GSM10039231GSM10039110GSM10039234GSM10039113GSM10039112GSM10039233GSM10039115GSM10039236GSM10039235GSM10039114GSM10039216GSM10039215GSM10039218GSM10039217GSM10039219GSM10039221GSM10039100GSM10039220GSM10039223GSM10039102GSM10039222GSM10039101GSM10039225GSM10039104GSM10039103GSM10039224GSM10039205GSM10039204GSM10039207GSM10039206GSM10039209GSM10039208GSM10039210GSM10039212GSM10039211GSM10039214GSM10039213GSM10039201GSM10039200GSM10039203GSM1003920224676346716Homo sapiens