{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346892/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346892"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Donor MHC class II mRNA combined with rapamycin programs Th1-like regulatory T cells and induces donor-specific alloimmune hyporesponsiveness","description":"Donor-specific immune tolerance remains a major goal in transplantation. In this study, we investigated whether donor major histocompatibility complex (MHC) class II could be delivered as an mRNA-encoded alloantigen to modulate donor-reactive T-cell responses. Using the B6.C-H2bm12–C57BL/6 model, mice were treated with donor MHC class II I-Aβ mRNA, rapamycin, or their combination. Donor MHC mRNA elicited alloimmune responses, whereas combination treatment promoted regulatory T-cell features and donor-specific hyporesponsiveness. Bulk RNA sequencing of splenic CD4+ T cells revealed a mixed effector- and regulatory-associated transcriptional response to donor MHC mRNA, with rapamycin modifying inflammatory transcriptional programs in a context-dependent manner.","dates":{"publication":"2026/09/15"},"accession":"GSE346892","cross_references":{"GSM":["GSM10042046","GSM10042047","GSM10042048","GSM10042049","GSM10042042","GSM10042043","GSM10042044","GSM10042045","GSM10042050","GSM10042051","GSM10042052","GSM10042041"],"GPL":["34290"],"GSE":["346892"],"taxon":["Mus musculus"]}}