GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347182/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347182GEOGSEfalseGradient-Free Isolation of Murine Pancreatic Islets for Single-Cell RNA SequencingPancreatic islet isolation for single-cell RNA sequencing (scRNA-seq) requires extensive enzymatic and mechanical processing that may affect cell recovery and transcriptional state. We evaluated a practical gradient-free workflow for murine pancreatic islets from diabetic BKS.Cg-Dock7^m +/+ Lepr^db/J mice. The protocol combined intraductal collagenase perfusion, controlled digestion, stereomicroscope-guided manual islet selection without density-gradient purification, and gentle Accutase/EDTA dissociation. Compared with Ficoll-based purification, the gradient-free workflow omitted density-gradient preparation, gradient centrifugation, additional wash centrifugations, and associated transfer steps while yielding viable cells suitable for scRNA-seq. The resulting library met the applied QC criteria and retained the major endocrine populations. Stress-associated transcriptional differences between the two pooled libraries were descriptive only. Overall, the gradient-free workflow represents a feasible alternative to Ficoll-based purification for preparing murine pancreatic islets for scRNA-seq.2026/09/30GSE347182GSM10047090GSM1004709124247347182Mus musculus[42776689]