{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347422/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Takifugu obscurus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347422"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A full-length transcriptome dataset of Takifugu obscurus for isoform-resolved analysis of complement C3 expression and splicing","description":"The anadromous pufferfish Takifugu obscurus is an economically important aquaculture species, yet comprehensive full-length transcriptome resources remain limited. Here, we generated an integrated transcriptomic dataset using PacBio Iso-Seq long-read sequencing and Illumina RNA-seq from 12 tissues. The PacBio dataset identified 23,116 isoforms corresponding to 12,173 genes, including 846 novel genes. Approximately 60% of genes were represented by a single isoform, while the remaining genes showed multiple isoforms with diverse transcript structures. Functional annotation identified transcripts associated with diverse biological processes and pathways, including immune-related functions. Three complement C3 genes located on different scaffolds were represented by 26, 9, and 6 isoforms, respectively, with most C3 isoforms showing predominant liver-associated expression. The resulting dataset provides full-length transcript sequences, structural and functional annotations, genomic coordinates, and tissue-associated expression information, supporting isoform characterization, alternative splicing studies, and comparative transcriptomic research in T. obscurus and other teleosts.","dates":{"publication":"2026/09/21"},"accession":"GSE347422","cross_references":{"GSM":["GSM10052660","GSM10052663","GSM10052664","GSM10052661","GSM10052662","GSM10052656","GSM10052657","GSM10052665","GSM10052654","GSM10052655","GSM10052658","GSM10052659"],"GPL":["37535"],"GSE":["347422"],"taxon":["Takifugu obscurus"]}}