GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347422/primaryOK200TranscriptomicsTakifugu obscurusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347422GEOGSEfalseA full-length transcriptome dataset of Takifugu obscurus for isoform-resolved analysis of complement C3 expression and splicingThe anadromous pufferfish Takifugu obscurus is an economically important aquaculture species, yet comprehensive full-length transcriptome resources remain limited. Here, we generated an integrated transcriptomic dataset using PacBio Iso-Seq long-read sequencing and Illumina RNA-seq from 12 tissues. The PacBio dataset identified 23,116 isoforms corresponding to 12,173 genes, including 846 novel genes. Approximately 60% of genes were represented by a single isoform, while the remaining genes showed multiple isoforms with diverse transcript structures. Functional annotation identified transcripts associated with diverse biological processes and pathways, including immune-related functions. Three complement C3 genes located on different scaffolds were represented by 26, 9, and 6 isoforms, respectively, with most C3 isoforms showing predominant liver-associated expression. The resulting dataset provides full-length transcript sequences, structural and functional annotations, genomic coordinates, and tissue-associated expression information, supporting isoform characterization, alternative splicing studies, and comparative transcriptomic research in T. obscurus and other teleosts.2026/09/21GSE347422GSM10052660GSM10052663GSM10052664GSM10052661GSM10052662GSM10052656GSM10052657GSM10052654GSM10052665GSM10052655GSM10052658GSM1005265937535347422Takifugu obscurus