{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE347nnn/GSE347518/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mycobacterium tuberculosis variant bovis BCG"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE347518"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of disA overexpression on BCG transcriptome","description":"This study investigates the transcriptomic consequences of graded intracellular cyclic di-AMP (c-di-AMP) production in Mycobacterium bovis BCG Danish 1331. Wild-type BCG Danish 1331 (BCG-WT) was used as the parental reference strain. Two recombinant strains overexpressing the Mycobacterium tuberculosis diadenylate cyclase gene disA (Rv3586) were generated. In rBCG-V1, disA is expressed from the episomal plasmid pSD5-hsp60 under the control of the constitutive hsp60 promoter. rBCG-V2 carries an extrachromosomal recombinant plasmid, pKSB-hsp60-disA, containing two tandem copies of the hsp60-disA expression cassette, together with duplicated kanR and oriM sequences, while the chromosomal cdnP locus remains intact. These engineered strains generate progressively elevated intracellular c-di-AMP levels, with rBCG-V2 exhibiting higher c-di-AMP production than rBCG-V1. RNA sequencing was performed to characterize the global transcriptional changes associated with increasing c-di-AMP levels by comparing rBCG-V1 and rBCG-V2 with the parental BCG-WT strain.","dates":{"publication":"2026/09/21"},"accession":"GSE347518","cross_references":{"GSM":["GSM10054609","GSM10054611","GSM10054610","GSM10054615","GSM10054614","GSM10054613","GSM10054612","GSM10054608","GSM10054607"],"GPL":["30387"],"GSE":["347518"],"taxon":["Mycobacterium tuberculosis variant bovis BCG"]}}