{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348126/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348126"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA sequencing of THP-1-derived macrophage-like cells with stable PGK1 overexpression versus empty vector control","description":"This project contains bulk transcriptomic data from THP-1 cells, a human monocyte cell line, that were differentiated into macrophage-like cells and used to model tumour-associated macrophages in triple-negative breast cancer (TNBC). THP-1 cells stably expressing either an empty vector or PGK1 were generated via lentiviral infection (pLVX constructs; puromycin selection). Cells were stimulated with 150 nM phorbol 12-myristate 13-acetate (PMA) for 24 h to induce differentiation into M0 macrophages, followed by 20 ng/mL IL-4 and IL-13 for 24 h, before RNA extraction. Total RNA was isolated with the RNAsimple Total RNA Kit (FOREGENE) and all six libraries were sequenced in a single batch at Lianchuan (Hangzhou, China).","dates":{"publication":"2026/09/23"},"accession":"GSE348126","cross_references":{"GSM":["GSM10066894","GSM10066897","GSM10066898","GSM10066895","GSM10066896","GSM10066899"],"GPL":["24676"],"GSE":["348126"],"taxon":["Homo sapiens"]}}