GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348126/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348126GEOGSEfalseBulk RNA sequencing of THP-1-derived macrophage-like cells with stable PGK1 overexpression versus empty vector controlThis project contains bulk transcriptomic data from THP-1 cells, a human monocyte cell line, that were differentiated into macrophage-like cells and used to model tumour-associated macrophages in triple-negative breast cancer (TNBC). THP-1 cells stably expressing either an empty vector or PGK1 were generated via lentiviral infection (pLVX constructs; puromycin selection). Cells were stimulated with 150 nM phorbol 12-myristate 13-acetate (PMA) for 24 h to induce differentiation into M0 macrophages, followed by 20 ng/mL IL-4 and IL-13 for 24 h, before RNA extraction. Total RNA was isolated with the RNAsimple Total RNA Kit (FOREGENE) and all six libraries were sequenced in a single batch at Lianchuan (Hangzhou, China).2026/09/23GSE348126GSM10066894GSM10066897GSM10066898GSM10066895GSM10066896GSM1006689924676348126Homo sapiens