GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348149/primaryOK200GenomicsSchizosaccharomyces pombeGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348149GEOGSEfalseTranscription termination safeguards quiescent chromatin for faithful cell-cycle re-entry.Quiescence is a conserved program of endurance and readiness in non-cycling cells that is fundamental to the longevity of eukaryotic lineages, from clonal microbial populations to human regenerative tissues. While this G₀ state is universally associated with a compact chromatin organization, the active safeguards that preserve this structural template—and their necessity for future division competence and fidelity—remain unknown. Here we show, using fission yeast as a model, that chromatin structural maintenance in quiescence requires the enforcement of transcription termination by the conserved factor Ppn1PNUTS/Ref2. We identify a minimal disordered region in Ppn1 that resolves a physical conflict in the quiescent genome by preventing the transcriptional “eviction” of cohesin. Loss of this safeguard drives a progressive structural erosion that deregulates cyclin dependent kinase (CDK) dynamics and causes lethal aneuploidy upon cell-cycle re-entry. Notably, this deterioration is not an inevitable terminal state; re-establishing termination via a brief Ppn1 pulse resets division fidelity by stabilizing a core de novo cohesin landscape. These findings redefine quiescent chromatin as an actively maintainable blueprint rather than a passive standby state established at G₀ entry. We propose that "quiescence exhaustion" is driven by transcriptional stress eroding genomic organization, defining a structural limit to cellular longevity.2026/09/25GSE348149GSM10067135GSM10067136GSM10067139GSM10067137GSM10067138GSM10067142GSM10067140GSM1006714128961348149Schizosaccharomyces pombe