{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348151/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Schizosaccharomyces pombe"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348151"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcription termination safeguards quiescent chromatin for faithful cell-cycle re-entry","description":"Quiescence is a conserved program of endurance and readiness in non-cycling cells that is fundamental to the longevity of eukaryotic lineages, from clonal microbial populations to human regenerative tissues. While this G₀ state is universally associated with a compact chromatin organization, the active safeguards that preserve this structural template—and their necessity for future division competence and fidelity—remain unknown. Here we show, using fission yeast as a model, that chromatin structural maintenance in quiescence requires the enforcement of transcription termination by the conserved factor Ppn1PNUTS/Ref2. We identify a minimal disordered region in Ppn1 that resolves a physical conflict in the quiescent genome by preventing the transcriptional “eviction” of cohesin. Loss of this safeguard drives a progressive structural erosion that deregulates cyclin dependent kinase (CDK) dynamics and causes lethal aneuploidy upon cell-cycle re-entry. Notably, this deterioration is not an inevitable terminal state; re-establishing termination via a brief Ppn1 pulse resets division fidelity by stabilizing a core de novo cohesin landscape. These findings redefine quiescent chromatin as an actively maintainable blueprint rather than a passive standby state established at G₀ entry. We propose that \"quiescence exhaustion\" is driven by transcriptional stress eroding genomic organization, defining a structural limit to cellular longevity.","dates":{"publication":"2026/09/25"},"accession":"GSE348151","cross_references":{"GSM":["GSM10067182","GSM10067171","GSM10067172","GSM10067183","GSM10067180","GSM10067181","GSM10067175","GSM10067186","GSM10067187","GSM10067176","GSM10067173","GSM10067184","GSM10067185","GSM10067174","GSM10067179","GSM10067188","GSM10067177","GSM10067178"],"GPL":["28961"],"GSE":["348151"],"taxon":["Schizosaccharomyces pombe"]}}