GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348495/primary200OKOtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348495GEOGSEfalseSpatially resolved single-cell transcriptomic profiling of human colorectal cancer using NanoString CosMx SMIThis study used the NanoString CosMx Spatial Molecular Imager (SMI) to perform spatially resolved single-cell transcriptomic profiling of FFPE human colorectal tissue. Seventeen samples were analyzed, including three colorectal polyp samples (CA), eleven stage I colorectal cancer samples (CCP1–CCP4), and three colorectal cancer-adjacent tissue samples (HCT). CosMx SMI enabled in situ detection and spatial localization of RNA transcripts at single-cell and subcellular resolution. The processed dataset includes per-cell gene expression matrices, transcript coordinates, cell metadata, field-of-view (FOV) positions, and cell segmentation polygon data, providing a resource for investigating spatial cellular heterogeneity and the tumor microenvironment in colorectal cancer. This study used the NanoString CosMx Spatial Molecular Imager (SMI) to perform spatially resolved single-cell transcriptomic profiling of FFPE human colorectal tissue. Seventeen samples were analyzed, including three colorectal polyp samples (CA), eleven stage I colorectal cancer samples (CCP1–CCP4), and three colorectal cancer-adjacent tissue samples (HCT). CosMx SMI enabled in situ detection and spatial localization of RNA transcripts at single-cell and subcellular resolution. The processed dataset includes per-cell gene expression matrices, transcript coordinates, cell metadata, field-of-view (FOV) positions, and cell segmentation polygon data, providing a resource for investigating spatial cellular heterogeneity and the tumor microenvironment in colorectal cancer.2026/09/30GSE348495GSM10073459GSM10073448GSM10073449GSM10073457GSM10073458GSM10073455GSM10073456GSM10073464GSM10073453GSM10073454GSM10073462GSM10073451GSM10073452GSM10073463GSM10073460GSM10073450GSM1007346133484348495Homo sapiens