{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348938/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348938"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA expression profiling of exosomes derived from human keloid fibroblasts under hypoxic and normoxic conditions","description":"This study aims to investigate the role of exosomal miRNAs derived from keloid fibroblasts (KFs) in promoting angiogenesis and keloid formation. Primary KFs were cultured under hypoxic (3% O2) and normoxic (20% O2) conditions, and exosomes were isolated from the conditioned medium. Small RNA sequencing was performed on these exosomes to identify differentially expressed miRNAs. A total of 6 samples were analyzed, including 3 biological replicates from the hypoxic group (D1-D3, Hypoxic-KFs-exo) and 3 from the normoxic group (C1-C3, Normoxic-KFs-exo). We identified 27 differentially expressed miRNAs, with miR-29a-3p significantly upregulated in hypoxic KFs-exo. Further functional and mechanistic studies revealed that exosomal miR-29a-3p is delivered into human umbilical vein endothelial cells (HUVECs), where it directly targets VASH1 to promote endothelial proliferation, migration, and tube formation, ultimately enhancing angiogenesis in keloid progression.","dates":{"publication":"2026/10/03"},"accession":"GSE348938","cross_references":{"GSM":["GSM10084061","GSM10084060","GSM10084059","GSM10084058","GSM10084057","GSM10084062"],"GPL":["16791"],"GSE":["348938"],"taxon":["Homo sapiens"]}}