GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE348nnn/GSE348938/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE348938GEOGSEfalseMicroRNA expression profiling of exosomes derived from human keloid fibroblasts under hypoxic and normoxic conditionsThis study aims to investigate the role of exosomal miRNAs derived from keloid fibroblasts (KFs) in promoting angiogenesis and keloid formation. Primary KFs were cultured under hypoxic (3% O2) and normoxic (20% O2) conditions, and exosomes were isolated from the conditioned medium. Small RNA sequencing was performed on these exosomes to identify differentially expressed miRNAs. A total of 6 samples were analyzed, including 3 biological replicates from the hypoxic group (D1-D3, Hypoxic-KFs-exo) and 3 from the normoxic group (C1-C3, Normoxic-KFs-exo). We identified 27 differentially expressed miRNAs, with miR-29a-3p significantly upregulated in hypoxic KFs-exo. Further functional and mechanistic studies revealed that exosomal miR-29a-3p is delivered into human umbilical vein endothelial cells (HUVECs), where it directly targets VASH1 to promote endothelial proliferation, migration, and tube formation, ultimately enhancing angiogenesis in keloid progression.2026/10/03GSE348938GSM10084061GSM10084060GSM10084059GSM10084058GSM10084057GSM1008406216791348938Homo sapiens