{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE349nnn/GSE349035/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE349035"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of NB4 cells treated with the PKM2 inhibitor PKM2-IN-1","description":"To investigate the transcriptional changes induced by PKM2 inhibition in acute myeloid leukemia (AML), we performed RNA sequencing (RNA-seq) in NB4 cells treated with the PKM2-specific allosteric inhibitor PKM2-IN-1. NB4 cells were treated with 0.25 μM PKM2-IN-1 or an equal volume of DMSO (control) for 48 h. Total RNA was isolated from three biological replicates per condition. Stranded RNA sequencing libraries were constructed and sequenced on the DNBSEQ-T7 platform in paired-end 150 bp mode. Raw reads were processed using Trimmomatic, and gene-level counts were obtained with featureCounts. Differentially expressed genes were identified using edgeR, and GSEA was performed to explore pathway enrichment. This dataset enables the identification of PKM2-regulated genes and provides insights into the role of PKM2 in AML cell metabolism and malignancy.","dates":{"publication":"2026/09/29"},"accession":"GSE349035","cross_references":{"GSM":["GSM10086084","GSM10086085","GSM10086082","GSM10086083","GSM10086080","GSM10086081"],"GPL":["29480"],"GSE":["349035"],"taxon":["Homo sapiens"]}}