{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE46nnn/GSE46158/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46158"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Microarray analysis of IdHP cells compared to LKS, Control ProB and E2A-/- progenitors","description":"Self-renewal potential and multipotency are hallmarks of the stem cell1-3. It is generally conceived that acquisition of such a stemness requires rejuvenation of somatic cells through reprogramming of genetic and epigenetic status. We show here that a simple blockage of cell differentiation is sufficient to make stem cells. By over-expression of transcriptional repressor Id3 in murine hematopoitic progenitor cells and cultivation of these cells in B cell induction conditions, cells exhibit developmental arrest and enter a self-renewal cycle. These cells can be maintained in vitro almost limitlessly, and the long-term cultured cells exhibited robust multi-lineage reconstitution when transferred into irradiated mice. These cells can be cloned and re-expanded with 50% of plating efficiency, indicating that virtually all cells are self-renewing. Equivalent progenitors were produced from progenitors in human cord blood. Thus produced stem cells will be useful as the source of cells for immune cell therapy.","dates":{"publication":"2014/06/01"},"accession":"GSE46158","cross_references":{"GSM":["GSM1125162","GSM1125164","GSM1125163","GSM1125165"],"GPL":["5642"],"GSE":["46158"],"taxon":["Mus musculus"]}}