GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE66nnn/GSE66936/primaryOK2000000GenomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE66936GEOGSE0falseExpression data from intermediate monocytes from healthy donors and autoimmune uveitis patientsHuman peripheral monocytes have been categorized into three subsets based on differential expression levels of CD14 and CD16. However, the factors that influence the distribution of monocyte subsets and the roles which each subset plays in autoimmunity are not well studied. To compare the gene expression profiling 1) on intermediate monocytes CD14++CD16+ monocytes between healthy donors and autoimmune uveitis patients and 2) among 3 monocyte subsets in health donors, here we purified circulating intermediate CD14++CD16+ monocytes from 5 patients with autoimmune uveitis (labeled as P1-5) and 4 healthy donors (labeled as HD1-4) by flow cytometry and isolated total RNA to proceed microarray assay. In addition, we also purified CD14+CD16++ (non-classical monocytes) and CD14++CD16- (classical monocytes) from 4 healthy donors to do microarray. We demonstrate that CD14++CD16+ monocytes from patients and healthy control donors share a similar gene expression profile. The CD14+CD16++ cells (non-classical monocytes) display the most distinctive gene expression profiling when compared to intermediate CD14++CD16+ monocytes and classical CD14++CD16- monocytes.2015/03/17GSE66936GSM1634600GSM1634611GSM1634610GSM1634599GSM1634598GSM1634597GSM1634596GSM1634595GSM1634594GSM1634608GSM1634607GSM1634606GSM1634605GSM1634604GSM1634603GSM1634614GSM1634613GSM1634602GSM1634612GSM1634601GSM163460957066936Homo sapiens[25911752]