GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE77nnn/GSE77970/primaryOK2000000GenomicsBurkholderia pseudomallei K96243Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77970GEOGSE0falseComparison of the transcriptomes of a Burkholeria pseudomallei bprR [bpss0688] mutant, a bprS [bpss0687] mutant and a bprRS double mutant with the wild-type B. pseudomallei K96243 parent strain.Objectives: The Burkholderia pseudomallei bprR and bprS genes are predicted to encode the response regulator and sensor kinase components respectively of a two component signal transduction system. Inactivation of either single component of this two component signal transduction system led to attenuation of B. pseudomallei virulence and also reduced motility. However, inactivation of both components resulted in wild-tpe virulence and motility. Therefore, we used RNA-seq to compare the transcritptomes of the three mutant strains (bprR single, bprS single, bprRS double mutant) with the wild-type parent strain in order to identify the genes differenty regulated in the different mutants.2016/04/27GSE77970GSM2062994GSM2063005GSM2063004GSM2062996GSM2062995GSM2062998GSM2062997GSM2062999GSM2063001GSM2063000GSM2063003GSM206300221459SRP07019277970Burkholderia pseudomallei K96243[27147217]