GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE81nnn/GSE81197/primaryOK2000000GenomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE81197GEOGSE0falseMacrophage Colony Stimulating Factor derived from CD4+ T cells aids in control of an intracellular infection [RNA-seq]Background: In malaria, parasites of the genus Plasmodium elicit robust host expansion of macrophages and monocytes, but the underlying mechanisms remain unclear. In a microarray analysis of pooled, activated CD4+ T cells from mice infected with P. chabaudi, we detected inducible expression of Csf1, which promotes macrophage proliferation. To better characterize Csf1-producing T cells, single-cell RNA-Seq was performed. Results: Robust Csf1 expression was detected in a subset of sampled CD4+ T cells (n = 14/35), whereas the remainder of cells had no detectable Csf1. Further, we identified ~ 400 genes that were differentially expressed between Csf1+ and Csf1- T cells. Conclusions: This work defines the transcriptional landscape of a subset of activated CD4+ T cells that produce the cytokine Csf1. These cells are expected to be important in infections with intracellular pathogens such as Plasmodium.2016/11/12GSE81197GSM2144443GSM2144442GSM2144441GSM2144440GSM2144447GSM2144446GSM2144445GSM2144444GSM2144429GSM2144428GSM2144449GSM2144427GSM2144448GSM2144461GSM2144460GSM2144454GSM2144432GSM2144431GSM2144453GSM2144452GSM2144430GSM2144451GSM2144458GSM2144436GSM2144457GSM2144435GSM2144434GSM2144456GSM2144433GSM2144455GSM2144439GSM2144438GSM2144437GSM2144459GSM214445017021SRP07458081197Mus musculus[27923070]