GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE8nnn/GSE8352/primaryOK2000000GenomicsMus musculusExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE8352GEOGSE0falseGeneration and purification of Th1 and Th2 effector and memory CD4+ T cellsNaïve CD4+ T cells were isolated from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice (Kaye et al., Nature 1989;341:746, Wright et al, Blood 2001;97:2278) that recognize a peptide of pigeon cytochrome C in the context of I-Ek and express CD44lo, CD62Lhi, CD45RBhi, and CD25-. After 4 days in vitro stimulation with antigen presenting cells (APC) under either Th1 or Th2 condition, naïve cells become Th1 or Th2 effector cells expressing CD44hi, CD62L lo, CD45RBhi, and CD25+. Additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-). These rested effector cells were adaptively transferred into thymectomized, lethally irradiated, and T cell depleted bone marrow reconstituted mice and memory cells were isolated after 4-12 weeks by flow sort. Keywords: Microarray analysis of Th1 and Th2 effector and memory CD4+ T cells2007/07/07GSE8352GSM207090GSM207059GSM207055GSM207077GSM207078GSM207056GSM207079GSM207057GSM207058GSM207095GSM207073GSM207074GSM207075GSM207076GSM207054GSM207091GSM207070GSM207092GSM207093GSM207071GSM207072GSM207094GSM207088GSM207066GSM207067GSM207089GSM207068GSM207069GSM207084GSM207062GSM207063GSM207085GSM207064GSM207086GSM207087GSM207065GSM207080GSM207081GSM207082GSM207060GSM207061GSM20708354288352Mus musculus[17724126]