{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE8nnn/GSE8528/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE8528"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Expression analysis of gene differentially expressed in the developping ovary","description":"In recent years, several factors required for follicle assembly and/or early growth of newly formed primordial follicles have been characterized, but it is likely that additional factors remain to be identified. We have used cDNA arrays to compare gene expression in the neonatal mouse ovary 48 h after birth (when primordial follicles are being assembled), and at 96 h (when early follicular growth is taking place), to that of ovaries collected less than 24 h after birth (when follicles have not yet been formed). Segregating genes according to their pattern of expression revealed the presence of one cluster of 24 genes whose expression consistently increased at 48 and 96 h. The top increaser in this cluster encodes a ~1.5 Kb mRNA containing an open reading frame of 1,401 bp that encodes a protein of 466 amino acids. The predicted 52.3 kDa protein, which we have termed Eop1 (for Early ovarian protein-1) is a novel member of the F-box only (Fbxo) protein family. Eop1 has a cytoplasmic localization that includes the endoplasmic reticulum. Eop1 mRNA expression is oocyte-specific; Eop1 mRNA is first detected on gestational day 18, decreasing thereafter to minimal levels on the day of birth. Eop1 mRNA prevalence increases again 48 and 96 h after birth, coinciding with the time of follicular assembly and the initiation of early follicular growth. The specific expression of Eop1 in oocytes and its developmental pattern of expression suggest a role for this novel gene in the regulation of oocyte physiology. Keywords: Time-course","dates":{"publication":"2007/11/21"},"accession":"GSE8528","cross_references":{"GSM":["GSM211702","GSM211703","GSM211704","GSM211699","GSM211700","GSM211701"],"GPL":["5369"],"GSE":["8528"],"taxon":["Mus musculus"],"PMID":["[18094359]","[22028443]"]}}