GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE8nnn/GSE8680/primaryOK2000000GenomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE8680GEOGSE0falseHEK and HeLa cells: transfection of siRNA directed against GFP versus mock transfectionWe tested the most widely used control siRNA directed against GFP for off-target effects and found by genome-wide expression profiling that it deregulates in addition to GFP a set of endogenous target genes. The detected modulated mRNA had target sequences homologous to the siRNA as small as 9 bp in size. However, we found no restriction of sequence homology to 3'UTR of target genes. Keywords: RNAi knock down2007/08/09GSE8680GSM215251GSM215250GSM215252GSM215246GSM215245GSM215248GSM215247GSM21524957048680Homo sapiens[18577207]