GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE89nnn/GSE89168/primaryOK2000000GenomicsDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE89168GEOGSE0falseTranscriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous systemTranscriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development.2017/03/21GSE89168GSM2359919GSM2359918GSM2359924GSM2359923GSM2359925GSM2359920GSM2359922GSM235992113304SRP09205489168Drosophila melanogaster[28195208]