GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE9nnn/GSE9059/primaryOK2000000GenomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE9059GEOGSE0falseMEF-CM and UM_GTA re-stimulation experiment with human ES cellsHuman embryonic stem cells can be maintained in a basic Serum Replacement (Invitrogen) based medium that has been conditioned on mouse embryonic fibroblasts (MEFs), yielding MEF-CM. Ligands secreted into the medium by the MEFs include Activin A, TGFß1, and Gremlin. This experiment served the purpose of identifying the short-term effects of MEF-CM and its substitute UM_GTA (unconditioned medium plus Activin A, TGFb1, and Gremlin) on gene expression in human embryonic stem cells. Keywords: Media / growth factor stimulation experiment2008/03/12GSE9059GSM229971GSM229970GSM22996927009059Homo sapiens[18393632]