GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE91nnn/GSE91063/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91063GEOGSEfalseSingle-cell Gene Expression Analysis Reveals Transcriptional Diversity among Human SpermatogoniaTwo individual human OCT4-positive spermatogonia were selected for global transcriptional capture using shallow RNA-seq. Successful cDNA synthesis and amplification was confirmed as reads mapped primarily to exons. It has to be noted though, that a comparision of cell to cell data is not possible as coverage of the whole transcriptome was too low, approximately <1.0x coverage. Nonetheless, RNA-seq data provided first information of highly abundant transcripts in individual human spermatogonia. Spermatogonia-specific expression of selected candidate genes with high RPKM values including DDX5, EEF1A1 and TSPY1 was confirmed by immunohistochemistry.2017/01/15GSE91063GSM2420384GSM242038317301SRP09479291063Homo sapiens[28093458]