GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE91nnn/GSE91381/primaryOK2000000GenomicsMus musculusExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE91381GEOGSE0falseDistinct populations of CD11b+ IRF4+ dendritic cells drive Th2 responses in the small intestine and colonWe have identified the dendritic cell (DC) populations that are sufficient for the induction of T helper 2 (Th2) cell responses in the intestine against both live Trichuris muris worms, and inert Schistosoma mansoni eggs. Antigen-specific Th2 responses did not develop after deletion of IRF4 in DCs, yet IRF4-deficient DCs were not functionally affected. Instead, IRF4flox/flox CD11c-cre mice had fewer CD11b+ migrating DCs, and fewer DCs carrying parasite antigen from the intestine. Adoptive transfer of purified DCs from infected animals directly into intestinal afferent lymphatics enabled us to identify that CD11b+CD103+ DCs were central to the induction of Th2 responses in the small intestine, whereas CD11b+CD103- DCs were more important in the colon. Similarly, after pulsing with Schistosome egg antigen (SEA) in vitro, adoptively-transferred small intestinal or colonic DCs acquired the ability to induce SEA-specific Th2 responses. These data demonstrate a functional specialisation among intestinal DC populations in inducing Th2 responses, and elucidate the roles of IRF4 in this process.2016/12/10GSE91381GSM2422315GSM2422316GSM2422305GSM2422306GSM2422307GSM2422311GSM2422312GSM2422313GSM2422314GSM2422310GSM2422308GSM24223092077591381Mus musculus[28598427]