GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v01/MSV000078923/primary200OK0000MetabolomicsBradley S. Moore6530 Quadrupole Time of Flight MS (Agilent Instrument)Lamellodysidea Herbaceahttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=a43f9a4ca3614be08ae6277dd8229363GNPS3nonePolyhalogenated dibenzo-p-dioxins are arguably among the most toxic molecules known to man. In addition to anthropogenic sources, marine invertebrates also harbor polybrominated dibenzo-p-dioxins of as yet unknown biogenic origin. Here, we report that the bmp gene locus in marine bacteria, a recently characterized source of polybrominated diphenyl ethers, can also synthesize dibenzo-p-dioxins by employing different phenolic initiator molecules. Our findings also diversify the structural classes of diphenyl ethers accessed by the bmp biosynthetic pathway. This report lays the biochemical foundation of a likely biogenetic origin of dibenzo-p-dioxins present in the marine metabolome and greatly expands the toxicity potential of marine derived polyhaloganated natural products.Polybrominated diphenyl ethers (PBDEs) and polybrominated bipyrroles are natural products that bioaccumulate in the marine food chain. PBDEs have attracted widespread attention because of their persistence in the environment and potential toxicity to humans. However, the natural origins of PBDE biosynthesis are not known. Here we report marine bacteria as producers of PBDEs and establish a genetic and molecular foundation for their production that unifies paradigms for the elaboration of bromophenols and bromopyrroles abundant in marine biota. We provide biochemical evidence of marine brominases revealing decarboxylative-halogenation enzymology previously unknown among halogenating enzymes. Biosynthetic motifs discovered in our study were used to mine sequence databases to discover unrealized marine bacterial producers of organobromine compounds.Biosynthesis of polybrominated aromatic organic compounds by marine bacteria.Enzymatic synthesis of polybrominated dioxins from the marine environment.Agarwal Vinayak V, El Gamal Abrahim A AA, Yamanaka Kazuya K, Poth Dennis D, Kersten Roland D RD, Schorn Michelle M, Allen Eric E EE, Moore Bradley S BSAgarwal Vinayak V, Moore Bradley S BS0falseGNPS_Lamellodysidea herbaceaSponge was collected at Anae Island in Guam by scuba.Wed Nov 05 13:35:00 GMT 2014MSV0000789232506197024974229279619