{"database":"GNPS","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v01/MSV000081381/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Metabolomics"],"submitter":["Max Cruesemann"],"instrument_platform":["micrOTOF-Q II"],"species":["Root-associated Bacteria"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=5deaf56894d748448f4143a81af8d7b0"],"submitter_email":["mcruesemann@gmail.com"],"submitter_affiliation":["University of Bonn"],"sample_protocol":[""],"repository":["GNPS"],"file_size":["865"],"ptm_modification":["no"],"data_protocol":[""],"pubmed_abstract":["Soil-dwelling microbes are the principal inoculum for the root microbiota, but our understanding of microbe-microbe interactions in microbiota establishment remains fragmentary. We tested 39,204 binary interbacterial interactions for inhibitory activities in vitro, allowing us to identify taxonomic signatures in bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified the antimicrobial 2,4-diacetylphloroglucinol (DAPG) and the iron chelator pyoverdine as exometabolites whose combined functions explain most of the inhibitory activity of the strongly antagonistic <i>Pseudomonas brassicacearum</i> R401. Microbiota reconstitution with a core of <i>Arabidopsis thaliana</i> root commensals in the presence of wild-type or mutant strains revealed a root niche-specific cofunction of these exometabolites as root competence determinants and drivers of predictable changes in the root-associated community. In natural environments, both the corresponding biosynthetic operons are enriched in roots, a pattern likely linked to their role as iron sinks, indicating that these cofunctioning exometabolites are adaptive traits contributing to pseudomonad pervasiveness throughout the root microbiota."],"pubmed_title":["Cofunctioning of bacterial exometabolites drives root microbiota establishment."],"pubmed_authors":["Getzke Felix F, Hassani M Amine MA, Crüsemann Max M, Malisic Milena M, Zhang Pengfan P, Ishigaki Yuji Y, Böhringer Nils N, Jiménez Fernández Alicia A, Wang Lei L, Ordon Jana J, Ma Ka-Wai KW, Thiergart Thorsten T, Harbort Christopher J CJ, Wesseler Hidde H, Miyauchi Shingo S, Garrido-Oter Ruben R, Shirasu Ken K, Schäberle Till F TF, Hacquard Stéphane S, Schulze-Lefert Paul P"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"GNPS_198 root bacteria+interactions","description":"Crude extracts of 198 root-associated bacteria, grown on TSB agar. Additionally, interaction zones of selected bacterial strains were extracted.\nAll file names contain essential information about the sample, first the strain number of the strain collection, then the extraction solvent (E=ethyl acetate, M= methanol). Filename 690_E.mzxml would correspond to strain 690, grown on TSB, extracted with ethyl acetate.\nFor measuring antibiotic interactions of two strains, halo zones of the agar were extracted the same way as for the wild type strains. Filenames depict first the antibioticall active strain, then the sensitive strain, and finally the extraction solvent. Filename 562480M.mzxml would thus correspond to strain 562 that was grown on a lawn of strain 480, its extraction zone was extracted with methanol.\n","dates":{"publication":"Tue Jul 25 01:07:00 BST 2017"},"accession":"MSV000081381","cross_references":{"pubmed":["37018204"]}}