GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v01/MSV000082463/primaryOK2000000MetabolomicsLuis Lopez-MolinaQ ExactivePseudomonas Aeruginosa (ncbitaxon:287)Arabidopsis Thaliana (ncbitaxon:3702)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=06f5cc94c68b44bba7fa97b4a5ffa8c8Luis.LopezMolina@unige.chUniversity of GenevaGNPS27MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetTo anticipate potential seedling damage, plants block seed germination under unfavorable conditions. Previous studies investigated how seed germination is controlled in response to abiotic stresses through gibberellic and abscisic acid signaling. However, little is known about whether seeds respond to rhizosphere bacterial pathogens. We found that <i>Arabidopsis</i> seed germination is blocked in the vicinity of the plant pathogen <i>Pseudomonas aeruginosa</i>. We identified L-2-amino-4-methoxy-trans-3-butenoic acid (AMB), released by <i>P. aeruginosa</i>, as a biotic compound triggering germination arrest. We provide genetic evidence that in AMB-treated seeds DELLA factors promote the accumulation of the germination repressor ABI5 in a GA-independent manner. AMB production is controlled by the quorum sensing system IQS. In vitro experiments show that the AMB-dependent germination arrest protects seedlings from damage induced by AMB. We discuss the possibility that this could serve as a protective response to avoid severe seedling damage induced by AMB and exposure to a pathogen.The plant pathogen <i>Pseudomonas aeruginosa</i> triggers a DELLA-dependent seed germination arrest in <i>Arabidopsis</i>.Chahtane Hicham H, Nogueira Füller Thanise T, Allard Pierre-Marie PM, Marcourt Laurence L, Ferreira Queiroz Emerson E, Shanmugabalaji Venkatasalam V, Falquet Jacques J, Wolfender Jean-Luc JL, Lopez-Molina Luis L0falseGNPS - Pseudomonas aeruginosa - Arabidopsis interactionSeed germination irreversibly transforms the plant (highly resistant?) embryo into a fragile seedling. To anticipate potential seedling damage seeds block their germination under unfavorable conditions. Historically, studies focused on how seeds respond to abiotic stresses such as canopy light or high temperature. They uncovered the central role of gibberellic (GA) and abscisic acid (ABA) signaling to control germination. Seeds however are continuously exposed to rhizosphere organisms, such as bacteria, acting as potential pathogens or commensals. Singularly little is known about seed germination control responses to biotic factors. We found that Arabidopsis seeds repress their germination in presence of the plant pathogen Pseudomonas aeruginosa in their environment. We identify L-2-amino-4-methoxy-trans-3-butenoic acid (AMB), released by P. aeruginosa, as the biotic compound triggering germination arrest. AMB production is controlled by the quorum sensing system IQS governing bacterial behavior. We show that AMB stimulates the activity of the DELLA factor RGL2 to promote the accumulation of ABI5, an ABA response bZIP factor and repressor of germination. We provide evidence that an AMB-dependent germination arrest protects the plant by avoiding severe seedling developmental perturbations induced by AMB and potential exposure to a pathogen.Wed Jun 13 08:12:00 BST 2018MSV00008246330149837