GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v02/MSV000085085/primaryOK2000000MetabolomicsLaura SanchezcompactimpactPseudomonas Psychrophila (ncbitaxon:122355)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=050edfa14f344319a33ee576a93b41a0sanchelm@uic.eduUniversity of Illinois at ChicagoGNPS12MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset0falseGNPS - 20200311_Pseudomonas_psychrophila_sp_JB418_MS2P. psychrophila sp. JB418 was grown on 10% Cheese Curd Agar (CCA) for 3 days prior to extraction. For extraction, Acetonitrile was added to each culture and sonicated for 1hr, then solvent was removed and dried to afford a dry weighed extract. Extracts were then dissolved in either 100% (pooled replicates) or 90% (individual replicates) Methanol and LC-MS/MS was performed. Pooled replicate was run on a Bruker Impact II qTOF and individual biological replicates were run on a Bruker compact qTOF. Solvent B was ACN with 0.1% Formic Acid and Solvent A was water with 0.1% Formic Acid for both analyses, gradient of 10-100% B over the course of 10-12 minutes on a UPLC C18 column. File naming: JB418_alone = pooled biological replicate, JB418 followed by number = individual biological replicates, CCA = media controls and MeOH = solvent blanks. Wed Mar 11 11:42:00 GMT 2020MSV000085085