{"database":"GNPS","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://massive-ftp.ucsd.edu/v03/MSV000087793/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Metabolomics"],"submitter":["Neha Garg"],"instrument_platform":["impact II"],"species":["Burkholderia Cenocepacia (ncbitaxon:95486)"],"full_dataset_link":["https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=faf86b2ed4044b22b99ad0a62c1fccc7"],"submitter_email":["ngarg42@gatech.edu"],"submitter_affiliation":["Georgia Institute of Technology"],"sample_protocol":[""],"repository":["GNPS"],"file_size":["299"],"ptm_modification":["MS:1002864 - No post-translational-modifications are included in the identified peptides of this dataset"],"data_protocol":[""],"pubmed_abstract":["Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how the chemical environment of CF lung influences the production of small molecule virulence factors by these strains. Here we compare metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium (SCFM2) and in a routine laboratory medium (LB), in the presence and absence of the antibiotic trimethoprim. Using a mass spectrometry-based untargeted metabolomics approach, we identify several compound classes which are differentially produced in SCFM2 compared to LB media, including siderophores, antimicrobials, quorum sensing signals, and various lipids. Furthermore, we describe that specific metabolites are induced in the presence of the antibiotic trimethoprim only in SCFM2 when compared to LB. Herein, C13-acyl-homoserine lactone, a quorum sensing signal previously not known to be produced by B. cenocepacia as well as pyochelin-type siderophores were exclusively detected during growth in SCFM2 in the presence of trimethoprim. The comparative metabolomics approach described in this study provides insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggests future work which could identify personalized strain-specific regulatory mechanisms involved in production of secondary metabolites. Investigations into whether antibiotics with different mechanisms of action induce similar metabolic alterations will inform development of combination treatments aimed at effective clearance of Burkholderia spp. pathogens."],"pubmed_title":["Metabolomic profiling of Burkholderia cenocepacia in synthetic cystic fibrosis sputum medium reveals nutrient environment-specific production of virulence factors."],"pubmed_authors":["Jaiyesimi Olakunle A OA, McAvoy Andrew C AC, Fogg David N DN, Garg Neha N"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"GNPS-Burkholderia_trimethoprim_SCFM2vsLB","description":"Infections by Burkholderia cenocepacia lead to life-threatening disease in immunocompromised individuals, including those living with cystic fibrosis (CF). While genetic variation in various B. cenocepacia strains has been reported, it remains unclear how environmental conditions found in the CF lung influence the production of small molecules that may impact virulence of these strains. Here we compare metabolomic profiles of three clinical B. cenocepacia strains cultured in synthetic CF sputum media (SCFM2) and in a routine laboratory media (LB), in the presence and absence of the antibiotic trimethoprim. Using a mass spectrometry based untargeted metabolomics approach, we identify several compound classes which are differentially produced in SCFM2 compared to LB media, including siderophores, antimicrobials, quorum sensing signals, and various lipid families. Furthermore, we describe how metabolomic changes induced by the antibiotic trimethoprim vary in SCFM2 compared to LB. The differences described in this study provide insight into the diversity of secondary metabolites produced by B. cenocepacia strains under conditions representative of the nutritional conditions present in CF-associated lung environments. ","dates":{"publication":"Sun Jul 11 20:50:00 BST 2021"},"accession":"MSV000087793","cross_references":{"pubmed":["34725378"]}}