GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v04/MSV000088673/primaryOK2000000MetabolomicsJoana Paula da Silva OliveiraGeisa Paulino Caprini EvaristoAndrea Furtado MacedoQ Exactive PlusCyrtopodium Glutiniferum (ncbitaxon:2019951)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=cd6521178d3b41e7955e65e545fc692bFiocruz Rond�nia/CEBIOUNIRIO - Federal University of the State of Rio de Janeirojoanapaulasoliveira@gmail.comgeisapc@gmail.comandrea.macedo@unirio.brGNPS73M+H, M-H<h4>Ethnopharmacological relevance</h4>Extracts of orchids have been traditionally used as human phytotherapeutics. Cyrtopodium flavum, for example, due to the analgesic and anti-inflammatory properties, beside the capacity of heal skin lesions has been focus of research. Also Cyrtopodium glutiniferum, an orchid found in the Brazilian southeastern rainforest, is known to synthesize anti-inflammatory glucomannans in the pseudobulbs, as other potentially therapeutic compounds.<h4>Aim of the study</h4>We have reported the first metabolomic analysis focused on the phenols expression of the neotropical orchid Cyrtopodium glutiniferum Raddi, besides free radical scavenging, anti-inflammatory and antiproliferative activities, and the genotoxicity properties of the aqueous extract.<h4>Material and methods</h4>The metabolomics of C. glutiniferum aqueous extract was performed through UHPLC-MSⁿ acquisition. We have detected the scavenging potential of the extract using DPPH assay. The genotoxic potential was performed by Ames Test (0-5000 μg mL^-1) and micronucleous assay (0-5000 μg mL^-1) in RAW264.7 cells. The cytotoxic potential of the extract against RAW264.7 was tested by WST-1 assay (0-500 μg mL^-1). And after all, the RAW264.7 cells were treated with non-cytotoxic concentrations of C. glutiniferum (0-50 μg mL^-1) to evaluate the antiproliferative and anti-inflammatory potential, besides the mitochondrial activity.<h4>Results</h4>From the 55 molecules identified, 45.5% belonged to the phenolic compounds database from Phenol Explorer, 29% to an in-house Orchidaceae molecules database, and 25.5% to both. Among the identified phenolic compounds, 18 subclasses were discriminated, being phenanthrenes the most abundant. Doses-dependent of C. glutiniferum extracts were able to induce DPPH free radicals scavenging and also to increase TA100 His⁺ revertants, in metabolic environment, showing mutagenicity just in the highest concentration, of 5 mg/plate. On Eukaryotic cell models, the extract also has induced dose-response and time-response cytotoxicity against RAW264.7 macrophages, mainly after 48 h and 72 h, even though the extract has not been able to induce the increase of micronucleated cells and mitotic index alteration on Micronucleus assay. The activation and proliferation of macrophages cultures were downregulated after 24 h and 48 h by the non-cytotoxic concentrations of the extract in a dose-dependent manner.<h4>Conclusions</h4>The Cyrtopodium glutiniferum metabolomics, anti-inflammatory and anti-proliferative properties observed in this study suggest a therapeutic efficacy of the orchid extract applied in folk medicine.Metabolomic analysis of Cyrtopodium glutiniferum extract by UHPLC-MS/MS and in vitro antiproliferative and genotoxicity assessment.Araújo-Lima Carlos Fernando CF, Paula da Silva Oliveira Joana J, Coscarella Isabella Leite IL, Aiub Claudia Alessandra Fortes CAF, Felzenszwalb Israel I, Caprini Evaristo Geisa Paulino GP, Macedo Andrea Furtado AF0falseGNPS Cyrtopodium glutiniferum Raddi_Atlantic ForestExtracts of orchids have been traditionally used as human phytotherapeutics. Cyrtopodium glutiniferum, an orchid found in the Brazilian southeastern rainforest, is known to synthesize anti-inflammatory and other potentially therapeutic compounds.Tue Jan 11 11:38:00 GMT 2022MSV00008867331982517