GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v06/MSV000093497/primaryOK200MetabolomicsWilton Ricardo Sala-CarvalhoHPLC-MS ESI low resolution (Thermo-Fischer)Brittonodoxa Subpinnatahttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=fa46caae090741f2b7b48fe508005e1ewiltonsala@hotmail.comUniversidade de Sao PauloGNPS77MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetfalseGNPS - Brittonodoxa subpinnata soluble extractFor HPLC-MS2 analysis, 50 mg of plant material was extracted with 5 mL of 80% methanol. The supernatant was collected and submitted to a cleanup process through a C18 SPE by activating the column with methanol and equilibrating it with ultrapure water. The extract was loaded into the column and eluted with 5 mL of the following solvents: ultrapure water, followed by 25% methanol, 50% methanol, and washed with methanol. The fractions were joined, transferred to a tube, and vacuum dried.Sat Nov 25 14:04:00 GMT 2023MSV000093497663595