GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v07/MSV000094717/primaryOK200MetabolomicsJason Crawford6550 iFunnel Q-TOF LC/MSHomo Sapiens (ncbitaxon:9606)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=7ac407b98d854632a04bae7025827e80jason.crawford@yale.eduYale UniversityGNPS23MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetfalseGNPS Human AKR1C3 Binds Agonists of GPR84 and Participates in an Expanded Polyamine PathwayIn vitro metabolomics with AKR1C3 and HEPG2 Metabolome Files and corresponding conditions: C3only: Enzyme (AKR1C3) only condition with NADPH co-factor added WTmed: this is medium only control containing HEPG2 extract and NADPH. WTmed+C3: this is a no-cofactor control containing enzyme and HEPG2 extract. WTmed+C3+PH: this is all components together with enzyme, HEPG2 extract and NADPH co-factor. Reversed-phase chromatography was performed with a Kinetex (Cat. # 00G-4601-E0) 5 um C18 100 A column (250 by 4.6 mm), using a water:acetonitrile gradient containing 0.1% formic acid at 0.7 mL/min flow rate: 0-30 min, 10% to 100% acetonitrile. Positive mode (qTOF)Wed May 08 12:19:00 BST 2024MSV000094717