GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v10/MSV000099005/primaryOK200MetabolomicsJeffrey M. BoydQ Exactive PlusStaphylococcus Aureus Subsp. Aureus Usa300_lachttps://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=48bae0936e054bf9bf7d603d136c390fjeffboyd@SEBS.Rutgers.eduRutgers, the State University of New JerseyGNPS243MS:1002864 - No post-translational-modifications are included in the identified peptides of this datasetfalseGNPS - An effective response to respiratory inhibition by a Pseudomonas aeruginosa excreted quinoline promotes Staphylococcus aureus fitness and survival in co-cultureTriplicate cultures of S. aureus USA300_LAC were cultured overnight in TSB before diluting to 0.1 (A600) in 5 mL of TSB in 25 mL culture tubes. Cells were grown for an additional 8 hours with 5 ug mL-1 Pseudomonas aeruginosa HQNO or vehicle control, after which 1 mL of each sample was centrifuged at 13,000g for 1 minute and washed once with PBS. Cell pellets were resuspended in 1 mL of an ice-cold 2:2:1 solution of Methanol:Acetonitrile:H2O. Harvested cells were lysed in a FastPrep homogenizer (MP Biomedicals) with 600 uL of 0.1 mm lysing matrix beads (MP Biomedicals) (2 cycles, 40 sec, 6.0 m sec-1). Samples were incubated on ice for 5 minutes before centrifuging twice at 13,000g for 2 minutes at 4C. A total of 850 uL of the supernatant was added to a spin filter and centrifuged again at 4C at 13,000g for 15 minutes. The samples were frozen at -80C until transferred to the metabolomics core of the Rutgers Cancer Institute of New Jersey for analysis.MSV000099005