GNPSapplication/xmlftp://massive-ftp.ucsd.edu/v11/MSV000099534/primaryOK200Metabolomicsyaseen ahmadThermo Q Exactive HF-X OrbitrapOryza Sativa (ncbitaxon:4530)https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f6f5349f1c034223a56a2e9dea852f68yaseena6@gmail.comGhent universityGNPS31nofalseGNPS - RAW data for untargeted metabolomics of rice shoot and root treated with PPEIn this study, shoot and root tissues from two-week-old rice plants, treated with peach peel extract (PPE) or mock solution, were harvested 24 hours post-treatment. Metabolites were extracted using 80% methanol with 0.1% formic acid, followed by sonication, centrifugation, and filtration. The extracts were analyzed using LC-MS on a Q Exactive Orbitrap system. Data were acquired in negative ion mode and processed using MS-DIAL software. Feature detection, deconvolution, and alignment were performed, and metabolites were annotated using public MSP spectral libraries. Quality control included pooled QC samples, solvent blanks, and mock-treated controls. This untargeted approach enabled the identification of metabolic shifts associated with PPE-induced systemic resistance in rice, providing insights into the biochemical pathways activated during defense responses.Sun Oct 19 02:28:00 BST 2025MSV000099534