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available"],"repository":["GPMDB"],"pubmed_abstract":["Epithelial-mesenchymal transition (EMT) describes a process whereby immotile epithelial cells escape structural constraints imposed by cellular architecture and acquire a phenotype characteristic of migratory mesenchymal cells. Implicated in carcinoma progression and metastasis, EMT has been the focus of several recent proteomics-based studies aimed at identifying new molecular players. To gain insights into extracellular mediators associated with EMT, we conducted an extensive proteomic analysis of the secretome from MDCK cells following oncogenic Ras-induced EMT (21D1 cells). Using Orbitrap technology and a label-free quantitative approach, differential expression of several secreted modulators were revealed. Proteomic findings were further substantiated by mRNA transcript expression analysis with 71% concordance. MDCK cells undergoing Ras-induced EMT remodel the extracellular matrix (ECM) via diminished expression of basement membrane constituents (collagen type IV and laminin 5), up-regulation of extracellular proteases (MMP-1, kallikreins -6 and -7), and increased production and secretion of ECM constituents (SPARC, collagen type I, fibulins -1 and -3, biglycan, and decorin). Collectively, these findings suggest that hierarchical regulation of a subset of extracellular effectors may coordinate a biological response during EMT that enhances cell motility. Transient silencing of MMP-1 in 21D1 cells via siRNA-mediated knockdown attenuated cell migration. Many of the secretome proteins identified broaden our understanding of the EMT process."],"pubmed_title":["Extracellular remodelling during oncogenic Ras-induced epithelial-mesenchymal transition facilitates MDCK cell migration."],"pubmed_authors":["Mathias Rommel A RA,Chen Yuan-Shou YS,Wang Bo B,Ji Hong H,Kapp Eugene A EA,Moritz Robert L RL,Zhu Hong-Jian HJ,Simpson Richard J RJ,","Mathias Rommel A RA, Chen Yuan-Shou YS, Wang Bo B, Ji Hong H, Kapp Eugene A EA, Moritz Robert L RL, Zhu Hong-Jian HJ, Simpson Richard J RJ"],"name_synonyms":["p21B, Transformation, Transitions, Epithelial Mesenchymal Transformation, c-Ha-ras, Ki-ras, Kras2, Epithelial-Mesenchymal Transformations, Hras-1, Epithelial Mesenchymal Transition, Madin-Darby canine kidney cell, H-ras, c-rasHa, K-ras, OCT3, Epithelial-Mesenchymal, epithelial-mesenchymal transition, MDCK, Madin-Darby canine kidney cell line., extracellular, ras, c-H-ras, EMT, mesenchymal cell differentiation from epithelial cell, Kras-2, AI929937, Transition, Epithelial-Mesenchymal Transformation, Ha-ras, Transformations, Harvey-ras, Epithelial-Mesenchymal Transitions, EMTH, Hras1"],"description_synonyms":["Tcsk, IGF-I, data, steel factor, Slf, Ki-ras, Organic cation transporter 3, hematopoietic growth factor KL, Kras2, Somatomedin-C, somatomedin, H-ras, c-rasHa, FPH2, K-ras, OCT3, electronic data file, MDCK, Cell, sKITLG, ras, mechano growth factor, Emt, Tsk, EMT, proteomic analysis, computer data file, MDCK Cells, Kras-2, Cell., AI929937, Igf-1, Ha-ras, EMTH, p21B, SHEP7, MDCK Cell, mast cell growth factor, c-Ha-ras, PSCTK2, Stem cell factor, Hras-1, somatomedin-C, SF, LPFS1, Kitl, STAT5, epithelial-mesenchymal transition, Mast cell growth factor, LYK, extracellular, stem cell factor, KL-1, c-H-ras, IGF1, MGF, c-Kit ligand, Cells, Mechano growth factor, mesenchymal cell differentiation from epithelial cell, KITLG, Soluble KIT ligand, Extraneuronal monoamine transporter, SCF, associated, Sl, Proteomes, Harvey-ras, Hras1"],"pubmed_title_synonyms":["p21B, Transformation, Transitions, Epithelial Mesenchymal Transformation, c-Ha-ras, Ki-ras, Kras2, Epithelial-Mesenchymal Transformations, Hras-1, Epithelial Mesenchymal Transition, Madin-Darby canine kidney cell, H-ras, c-rasHa, K-ras, OCT3, Epithelial-Mesenchymal, epithelial-mesenchymal transition, MDCK, Madin-Darby canine kidney cell line., extracellular, ras, c-H-ras, EMT, mesenchymal cell differentiation from epithelial cell, Kras-2, AI929937, Transition, Epithelial-Mesenchymal Transformation, Ha-ras, Transformations, Harvey-ras, Epithelial-Mesenchymal Transitions, EMTH, Hras1"],"pubmed_abstract_synonyms":["projections, Transitions, lamina densa, Epithelial Cell, Metastasis, Organic cation transporter 3, basement membrane of connective tissue, Epithelial Mesenchymal Transition, Malignant Epithelial Tumors, c-rasHa, Neoplasm Metastases, Glandular, Tumor, organ process., Epithelial Tumors, MDCK, Transcript Expression Analysis, Procollagen Type IV, Readability, ras, \"epithelial carcinoma\" EXACT [NCI2004_11_17:C2916], alpha2 Subunit, responsivity, symptoms, Gene Expression Monitorings, MDCK Cells, Basal Laminas, no subtype (morphologic abnormality)\" EXACT [SNOMEDCT_2005_07_31:68453008], MMP-1, cell movement, Profilings, Analysis, Type IV Collagen, Extracellular Matrices, Carcinomatosis, Laminin M, increased, Spindle-Cell Carcinoma, MDCK Cell, Undifferentiated, Collagen alpha2(I), Analyses, Kininogenase, Adenomatous Epithelial, proteins, free, INSDC_feature:ncRNA, 22 kDa interstitial collagenase, Lamina, LYK, INSDC_qualifier:siRNA, GPIa*, alpha1(IV) collagen, alpha2 Chain, papilla, Type 1 Collagen, Kallikrein Light Chain, basal lamina, Collagen, associated, beta Kallikrein, Display, Transitional Epithelial Cell, screening, Bone Proteoglycan I, Carcinoma, Basement, anatomical protrusion, Undifferentiated Carcinoma, small interfering RNA, Ki-ras, external secretion, Arts, lamina, \"carcinoma\" EXACT [CSP2005:2000-1867], Monitorings, Up-Regulation (Physiology), flanges, Adenomatous, Laminin, beta-Kallikrein, \"carcinoma, Epithelial-Mesenchymal, Squamous, Transitional Epithelial, Anaplastic, Gene Expression, exocrine gland fluid/secretion, Kallikrein B', Type I Collagen, Epitheliomas, count, Collagen Type I, Callicrein, EMT, Emt, shelf, Kallidinogenase, concluding remarks, secretion, M Chain, Basement Membranes, alpha1 Type 1 Collagen, Transformations, Epithelial-Mesenchymal Transitions, Carcinomatoses, Kallikrein A, Carcinomas, Adenomatous Epithelial Cell, Adenomatous Epithelial Cells, Squamous Cell, Industrial, Spindle Cell, Industrial Arts, protein_coding_transcript, Displays, c-Ha-ras, Upregulation, Transcriptome Profilings, PSCTK2, shelves, conclusion, Basal, Merosin, Hras-1, signs, mRNA Differential, DSPG-II, projection, ridge, Type 1, summary, Phenotypes, Kalliginogenase, spine, c-H-ras, Spindle-Cell, Cells, Malignant Epithelial Neoplasm, Laminin M Chain, beta-Kallikrein B, Anaplastic Carcinomas, 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remodelling during oncogenic Ras-induced epithelial-mesenchymal transition facilitates MDCK cell migration.","description":"PeptideAtlas ID: PAe003680. Original data file: O070224-30.mgf. Data published as part of J Proteome Res. 2010 Feb 5;9(2):1007-19  . From the Abstract: {{i}}... To gain insights into extracellular mediators associated with EMT, we conducted an extensive proteomic analysis of the secretome from MDCK cells following oncogenic Ras-induced EMT (21D1 cells). ...{{/i}}","dates":{"submission":"2012-07-21"},"accession":"GPM11210000341","cross_references":{"pubmed":["19954229"]}}