{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":27,"searchCount":3},"additional":{"omics_type":["Other"],"submitter":["Urbaniak MD, et al."],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Trypanosoma_brucei_ncbi"],"publication":["22574199"],"submitter_mail":["Not available"],"submitter_affiliation":["Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003131","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003130","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003122","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003124","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003123","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003126","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003125","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003128","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003127","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003129"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host and an insect vector, and adaption of its proteome between lifecycle stages is essential to its survival and virulence. We have optimized a procedure for growing Trypanosoma brucei procyclic form cells in conditions suitable for stable isotope labeling by amino acids in culture (SILAC) and report a comparative proteomic analysis of cultured procyclic form and bloodstream form T. brucei cells. In total we were able to identify 3959 proteins and quantify SILAC ratios for 3553 proteins with a false discovery rate of 0.01. A large number of proteins (10.6%) are differentially regulated by more the 5-fold between lifecycle stages, including those involved in the parasite surface coat, and in mitochondrial and glycosomal energy metabolism. Our proteomic data is broadly in agreement with transcriptomic studies, but with significantly larger fold changes observed at the protein level than at the mRNA level."],"pubmed_title":["Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages."],"pubmed_authors":["Urbaniak Michael D MD,Guther M Lucia S ML,Ferguson Michael A J MA,","Urbaniak Michael D MD, Guther M Lucia S ML, Ferguson Michael A J MA"],"name_synonyms":["Trypanosoma (Trypanozoon) brucei, proteomic analysis, Trypanosoma brucei subgroup."],"description_synonyms":["IGF-I, Intervention Strategies, negative regulation of adrenergic receptor signalling pathway, data, Beliefs, steel factor, Trypanosoma brucei subgroup, Slf, hematopoietic growth factor KL, Aminokarbonsaeure, Aminosaeure, Proteins, Somatomedin-C, Aminocarbonsaeure, somatomedin, Gene, FPH2, Trypanosoma (Trypanozoon) brucei, ratio., Procedure, Cultural Backgrounds, electronic data file, Isotope-Coded Affinity, Labeling, Cell, sKITLG, Intervention or Procedure, Belief, polypeptide, Isotope, Background, Cultural Background, Cultures, Stable Isotope, mechano growth factor, Cultural, Protein, proteomic analysis, Gene Products, computer data file, Isotopically-Coded Affinity, Igf-1, Isotope Coded Affinity Tagging, Isotopically-Coded Affinity Tagging, Backgrounds, Intervention, SHEP7, proportion, amino acids, mast cell growth factor, interventionDescription, Stem cell factor, proportionality, somatomedin-C, SF, Interventional, Kitl, rate, proteins, Isotope Labeling, STAT5, Amino, Affinity Tagging, Mast cell growth factor, Isotopically-Coded, SURGICAL AND MEDICAL PROCEDURES, Protein Gene Products, Gene Proteins, stem cell factor, KL-1, false, Stable, Custom, IGF1, MGF, c-Kit ligand, regulation of adrenergic receptor signalling pathway, Mechano growth factor, Isotope-Coded Affinity Tagging, quotient, Isotope-Coded, Customs, KITLG, Acids, Soluble KIT ligand, SCF, Tagging, Sl, Stable Isotope Labeling"],"pubmed_title_synonyms":["Trypanosoma (Trypanozoon) brucei, proteomic analysis, Trypanosoma brucei subgroup."],"pubmed_abstract_synonyms":["big, host organism, negative regulation of adrenergic receptor signalling pathway, Beliefs, Trypanosoma brucei subgroup, Ass-1, Metabolisms, Aminosaeure, mRNA., Aminocarbonsaeure, number, Gene, Bioenergetics, Trypanosoma (Trypanozoon) brucei, Isotope-Coded Affinity, Intervention or Procedure, Background, large, surface, Metabolism, AA408052, Cultural, Gene Products, fold, Isotopically-Coded Affinity, Expenditure, Isotopically-Coded Affinity Tagging, Backgrounds, integumentum commune, viral infection, proportion, amino acids, interventionDescription, Interventional, Energy, messenger RNA, virus process, proteins, number of, Expenditures, SURGICAL AND MEDICAL PROCEDURES, ASS, template RNA, Stable, Custom, time of survival, great, has or lacks parts of type, Isotope-Coded Affinity Tagging, Customs, Tagging, Stable Isotope Labeling, ratio, Intervention Strategies, data, external covering of organism, Aminokarbonsaeure, Proteins, organism surface, extra or missing physical or functional parts, Procedure, Cultural Backgrounds, Labeling, Cell, Belief, mereological quality, polypeptide, Isotope, Cultural Background, survival, Cultures, Stable Isotope, Protein, proteomic analysis, Pathogenicity, INSDC_feature:mRNA, Insects, Isotope Coded Affinity Tagging, Energy Expenditure, Intervention, protein_coding_transcript, death rate, body surface, proportionality, expanded, Energy Expenditures, rate, Isotope Labeling, Amino, Affinity Tagging, Isotopically-Coded, Insect, Bioenergetic, Protein Gene Products, Gene Proteins, Energy Metabolisms, Parasite, false, enlarged, integumentary system, regulation of adrenergic receptor signalling pathway, cardinality, quotient, Isotope-Coded, virulence, Acids, dermal system, Proteomes"],"view_count":["27"],"citation_count":["0"],"search_count":["3"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM11210003129"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","varsite~0"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.008328192473781616"],"download_count_scaled":["0.0"],"reanalysis_count":["0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Comparative SILAC proteomic analysis of Trypanosoma brucei bloodstream and procyclic lifecycle stages.","description":"RANCHE KEY: 6rtELh46IFS7gExEGuFR7E2t3UZABvuzvkh0U5sz71SSZM+XogXG2a8Omdg5+KWQCmjpP30xZWhujBngVbaqsT9ZTpYAAAAAAAAGCw== Original data file: BSF-F3-7.mgf. Data published as part of PLoS One. 2012;7(5):e36619  . From the Abstract: {{i}} ... We have optimized a procedure for growing Trypanosoma brucei procyclic form cells in conditions suitable for stable isotope labeling by amino acids in culture (SILAC) and report a comparative proteomic analysis of cultured procyclic form and bloodstream form T. brucei cells. In total we were able to identify 3959 proteins and quantify SILAC ratios for 3553 proteins with a false discovery rate of 0.01 ...{{/i}}","dates":{"submission":"2012-08-09"},"accession":"GPM11210003129","cross_references":{"pubmed":["22574199"]}}